Validation of the SPF 10 LiPA Human Papillomavirus Typing Assay Using Formalin-Fixed Paraffin-Embedded Cervical Biopsy Samples

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray

A formalin-fixed paraffin-embedded (FFPE) sample usually yields highly degraded DNA, which limits the use of techniques requiring high-quality DNA, such as Infinium Methylation microarrays. To overcome this restriction, we have applied an FFPE restoration procedure consisting of DNA repair and ligation processes in a set of paired fresh-frozen (FF) and FFPE samples. We validated the FFPE result...

متن کامل

Deep Clonal Profiling of Formalin Fixed Paraffin Embedded Clinical Samples

Formalin fixed paraffin embedded (FFPE) tissues are a vast resource of annotated clinical samples. As such, they represent highly desirable and informative materials for the application of high definition genomics for improved patient management and to advance the development of personalized therapeutics. However, a limitation of FFPE tissues is the variable quality of DNA extracted for analyse...

متن کامل

Detection and toxin typing of Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples by PCR.

Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a PCR assay to detect toxin-encoding genes and the 16S rRNA gene of C. perfringens. We successfully detected and genotyped C. perfringens in tissue sections from two autopsy cases.

متن کامل

Detection of papillomavirus DNA in formalin-fixed paraffin-embedded equine aural plaque samples

Equine aural plaques were recently associated with four equine papillomaviruses (EcPVs) (Lange et al., 2013; Taniwaki et al., 2013). These viruses have been detected in fresh samples by PCR (Lange et al. 2013; Taniwaki et al., 2013), but EcPVs have not been identified in formalinfixed and paraffin-embedded (FFPE) aural plaque samples. Archives of FFPE aural plaques and other papilloma specimens...

متن کامل

Improvement of DNA Extraction for Human Papillomavirus Genotyping from Formalin-Fixed Paraffin-Embedded Tissues

Mucosal human papillomaviruses (HPVs) play a crucial role in the development of cervical carcinoma, the third most common malignancy in women worldwide. These viruses are classified as high risk (HR) or low risk (LR), depending on their transforming ability. Formalinfixed paraffin-embedded (FFPE) tissues represent the most frequent form of tissue storage in pathology departments. These archival...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 2009

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.00286-09